Benzylaminopurine in short form BAP is a type of cytokinin supplement for plant growth and development.
It helps increase the post-harvest life of green vegetables by preventing chlorophyll degradation.
1. To prepare a 1 mg/ml stock solution, add 100 mg of the Benzylaminopurine to a 100 ml volumetric flask or glass container.
2. Add 2-5 ml of 1 N NaOH to dissolve the powder. Once completely dissolved, bring to volume with molecular biology-grade water. Stirring the solution while adding water may be required to keep the material in the solution.
3. Store the stock solution at 2 - 8 °C.
4. Add 1.0 ml of the stock solution to 1 liter of medium to obtain a final concentration of 1.0 mg/L of the Benzylaminopurine in the culture medium.
Note: Benzylaminopurine is generally used in plant cell culture at a concentration of .1 – 10 mg/L. When added in appropriate concentrations Benzylaminopurine can stimulate auxiliary and adventitious root formation.