Agar is a jelly-like substance, obtained from red algae, used heavily as a solid medium for growing various microbes. Many specific formulations are available because some microbes prefer certain environmental conditions over others.
Burkholderia Cepacia Medium - A medium for the selective isolation of Burkholderia cepacia from the respiratory secretions of patients with cystic fibrosis and for routine testing of non-sterile inorganic salt solutions containing a preservative.
Preparation:
- Suspend 18.25 g in 500 ml of distilled water.
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Mix well and sterilize by autoclaving at 121oC for 15 minutes.
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Cool to 50oC and aseptically add the contents of one vial of Burkholderia cepacia Selective Supplement (SR189E) reconstituted as directed.
- Mix well and pour into sterile petri dishes.
Typical Formula (g/l)
pH 6.2 ± 0.2 at 25oC
Peptone 5.0; Yeast extract 4.0; Sodium pyruvate 7.0;
Potassium dihydrogen phosphate 4.4;
Di-sodium hydrogen phosphate 1.4; Bile salts 1.5;
Ammonium sulphate 1.0; Magnesium sulphate 0.2;
Ammonium ferrous sulphate 0.01; Phenol red 0.02;
Crystal violet 0.001; Agar 12.0.
