Agar is a jelly-like substance, obtained from red algae, used heavily as a solid medium for growing various microbes. Many specific formulations are available because some microbes prefer certain environmental conditions over others.
EE Broth, Mossel - an enrichment medium for bile-tolerant Gram-negative bacteria in the microbiological examination of pharmaceutical products
Directions: Suspend 43.5 grams in 1000 ml distilled water. Dispense 120 ml amounts in 250 ml flasks or 9 ml amounts in tubes. Stopper with cotton plugs or loose fitting caps. Heat in free flowing steam or boiling water for 30 minutes. Avoid overheating of the medium.
DO NOT AUTOCLAVE.
Standard Formula
Ingredients Gms/litre
Peptic digest of animal tissue 10.00
Dextrose 5.00
Ox-bile, purified 20.00
Disodium phosphate 6.45
Monopotassium phosphate 2.00
Brilliant green 0.0135
Final pH (at 25oC) 7.2 ± 0.2
