Mannitol Salt Agar - A selective medium for the isolation of presumptive pathogenic staphylococci. Most other bacteria are inhibited, with the exception of a few halophilic species.
Preparation:
- Disperse 108g in 1L of deionized water.
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Soak for 10 minutes, swirl to mix, and sterilize by autoclaving for 15 minutes at 121oC.
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Cool to 47oC and mix before pouring into Petri dishes and then dry the agar surface.
Typical g/L
pH: 7.4 ± 0.2
- Beef extract 1.0,
- Balanced Peptone No. 1 10.0.
- Sodium Chloride 75.0
- D-Mannitol 10.0
- Agar No. 2 12.0
- Phenol Red 0.025.
