Agar is a jelly-like substance, obtained from red algae, used heavily as a solid medium for growing various microbes. Many specific formulations are available because some microbes prefer certain environmental conditions over others.
Pseudomonas Agar F Base - For the selective isolation of Pseudomonas species
Preparation: Dissolve 35 g/l together with 10 ml/l of glycerol (MERCK, Cat. No. 4091) in demin water by heating in a boiling water bath or in a current of steam; if required, dispense into tubes; autoclave (15 min. at 121oC). Make slant agar tubes or pour plates.
pH: 7.2 ± 0.1 at 25oC.
Typical composition (g/litre): Peptone from casein 10.0; Peptone from meat 10.0; Magnesium sulfate 15; Potassium hydrogen phosphate 1.5; Agar-agar 12.0