Agar is a jelly-like substance, obtained from red algae, used heavily as a solid medium for growing various microbes. Many specific formulations are available because some microbes prefer certain environmental conditions over others.
Reinforced Clostridial Agar - A solid medium for the cultivation and enumeration of anaerobes, especially Clostridium species.
Directions: Suspend 51.0 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121oC) for 15 minutes.
Standard Formula
Ingredients Gms/litre
Casein enzymic hydrolysate 10.00
Yeast extract 3.00
Beet extract 10.00
Dextrose 5.00
Sodium chloride 5.00
Sodium acetate 3.00
Starch soluble 1.00
L-Cysteine hydrochloride 0.50
Agar 13.50
Final pH (at 25oC) 6.8 ± 0.2
