Agar is a jelly-like substance obtained from red algae, used heavily as a solid medium for growing various microbes.
Many specific formulations are available because some microbes prefer certain environmental conditions over others.
Preparation:
- Suspend 48 g in 1 liter of demin water by heating in a boiling water bath
-
Autoclave carefully (10 min. at 121oC). Do not overheat
pH: 5.6 ± 0.2 at 25oC.
Typical composition (g/litre):
- Malt extract 30.0
- Peptone from soymeal 3.0
- Agar-agar 15.0
